Abcam homepage

Strap Ankle Chunky Size 39 Color for amp; Black Sandals Comfort Evening Party Open C Women's Toe Buckle Shoes Leather Heel Summer Dress xqtItA

Leather Evening Strap 39 Comfort Heel C Size Buckle Dress Black for Shoes Color Sandals Women's amp; Chunky Party Ankle Open Summer Toe

All tags TAOFFEN Shoes Party Gold Court Stiletto Women's rfxOq6UrwPrimary antibodies Knockout (KO) validated antibodies: specificity confirmed

Knockout (KO) validated antibodies: specificity confirmed

Cognac Snowflake III Trotters Boot Women's w7gSYS

With the increasing need for highly specific antibodies, Abcam introduces KO validation as a standard quality control protocol for antibodies at scale


The need

Affinity binders/antibodies are amongst the most commonly used tools in life science to study proteins and their functions within various biological pathways and diseases. With their specificity and sensitivity, these tools can help to easily identify the protein of interest at various expression levels. However, an increasing number of studies have shown that not all of these tools are specific, leading to a growing issue of experimental irreproducibility. This irreproducibility is leading to wasted time, resources and, unfortunately, the retraction of papers within the scientific community (Bradbury & Plückthun, 2015; Baker, 2015; Mullane et al., 2015701B Platform Black Sandal Pleaser Taboo Women's Patent Black 7qnREg1x).

The solution: knockout validation

One of the most accepted and trusted validation processes for antibody specificity is through knockout validation (Madhusoodanan, 2014; Rhodes & Trimmers, 2008). In a knockout validation, antibody specificity is confirmed by testing the antibody of interest on a knockout sample or cell line which doesn't express the target protein.  In a knockout cell line, the antibody target is not present as the gene encoding the protein of interest is eliminated or "knocked out". This data is compared side-by-side against a normal cell line (or wild type) and if the antibody is truly specific, the antibody should have no detection in the knockout cell line, but instead a specific signal in the normal cell line. Knockout validations offers a true negative control to confirm the antibody specificity to the protein of interest (Bordeaux, et al., 2010).

Knockout validation as a part of Abcam's standard antibody validationShoes Sneakers Women's shoes Grey Fashion Running Walking Casual Lightweight xqBHq10UA

Abcam has incorporated knockout based validation as a standard level of validation for its antibody products. We are starting at 500 antibodies a year, and we will knockout validate these antibodies as a part of our rigorous antibody validation process.

When you see the knockout validated seal, you can trust that the antibody has not only been validated in the recommended applications and species, but its specificity has been confirmed through our in-house knockout validation approach.

Abcam knockout validates its antibodies using the extensive library of knockout cell lines available from Horizon Discovery.


  • See how KO validation compares to other types of antibody validation assays

  • Learn more about the technology behind our KO cell lines

  • See different scenarios of results from KO validation 
for Dress Party Sandals amp; Comfort Heel Ankle Open Strap Color Evening Women's Buckle Black C 39 Leather Chunky Toe Size Summer Shoes
See which antibodies have been knockout validated


We want to hear from you

If you have any comments or questions, please feel free to contact us by phone or email, or by simply filling out an online inquiry form.




​References​​​​​​​

Strap Ankle Chunky Size 39 Color for amp; Black Sandals Comfort Evening Party Open C Women's Toe Buckle Shoes Leather Heel Summer Dress xqtItA Strap Ankle Chunky Size 39 Color for amp; Black Sandals Comfort Evening Party Open C Women's Toe Buckle Shoes Leather Heel Summer Dress xqtItA Strap Ankle Chunky Size 39 Color for amp; Black Sandals Comfort Evening Party Open C Women's Toe Buckle Shoes Leather Heel Summer Dress xqtItA Strap Ankle Chunky Size 39 Color for amp; Black Sandals Comfort Evening Party Open C Women's Toe Buckle Shoes Leather Heel Summer Dress xqtItA
  • Bradbury A, Plückthun A (2015). Reproducibility: Standardize antibodies used in research. Nature 518(7537):27-9. doi: 10.1038/518027a.
  • Baker M (2015) Reproducibility crisis: Blame it on the antibodies. Nature 521(7552):274-6. doi: 10.1038/521274a.
  • Mullane K, et al., (2015). Guidelines for manuscript submission in the peer-reviewed pharmacological literature. Biochem Pharmacol. pii: S0006-2952(15)00358-5. doi: 10.1016/j.bcp.2015.06.023.
  • Madhusoodanan J (2014) .  Validating Antibodies: An Urgent Need.  The Scientist.
  • Rhodes KJ, Trimmer JS (2008). Antibody-based validation of CNS ion channel drug targets. J Gen Physiol. 131(5):407-13. doi: 10.1085/jgp.200709926.
  • Bordeaux, et al., (2010).​ Antibody validation. Biotechniques 48(3):197-209. doi: 10.2144/000113382.​​​


Brown Crazy Women Work John JD3594 Boot 5 M PU Dark Horse Deere 5 IqHIwP
Get resources and offers direct to your inbox Sign up